Landing pad plasmid maps

I still intend to get around to posting most if not all landing pad plasmids to Addgene, but it’s taken me forever to get around to it. Actually, the institution takes just as forever to do it, since apparently they have to get permissions to post any plasmid with parts I may have amplified from another source(eg. the puromycin resistance gene)? Once those are there, the plasmid sequences will obviously be publicly available. In the mean time, I figure I’d post some of the most common plasmid maps here, so other people can benefit form them (or I don’t have to send specific emails to each person who asks for them). So here are some of the most popular, published plasmids, in GenBank (.gb) format.

G542A_pLenti-Tet-coBxb1-2A-BFP_IRES_iCasp9-2A-Blast_rtTA3 (Addgene #171588)

G698C_AttB_ACE2_IRES_mCherry-H2A-P2A-PuroR (Addgene #171594)

G758A_AttB_ACE2(del)-IRES-mCherry-H2A-P2A-PuroR (Addgene #171596)

p0489_AttB_EGFP-Link-PTEN-IRES-mCherry_Bgl2.gb

p0669_AttB_sGFP-PTEN-IRES-mCherry-P2A-bGFP_Bgl2

G384A_LLP-iCasp9-Blast

G273A_AttB_sGFP-PTEN-IRES-mCherry-P2A-HygroR

G274A_attB-sGFP-PTEN_IRES-mCherry-P2A-PuroR

attB-EGFP (Addgene #171597)

attB-mCherry (Addgene #171598)

dAAVS1_TetBxb1_mTagBFP2_rtTa3

dAAVS1_CMV-rtTA3-Term

dAAVS1_Dummy

G371A_LLP-rEF1a

G375F_attB-eGFP-rIRES-mCherry

G382C_attB-eGFP-rIRES-mCherry-2A-PuroR

G163A/B_AttB_PuroR-P2A-mCherry_Bgl2

G310A_pLenti-TetBxb1BFP-2A-coBxb1-2A-Blast_rtTA3

pLenti-TetBxb1BFP_coBxb1-rtTA3_Blast

pLenti-TetBxb1BFP-rtTA3_Blast

G57A_attB_TP53-link-EGFP-IRES-mCherry

AttB_sGFP-VHL-IRES-mCherry-P2A-bGFP_Bgl2

AttB_VHL-sGFP_IRES-mCherry-P2A-bGFP_Bgl2