So I had collected this data over 10 months ago, when I made an initial foray into trying the fluorogen activating peptides dL5 in the presence of Malachite Green, a dirt cheap small molecule (You can use it to try to kill fungus eating at your fish in your aquarium). A recent email reminded me about fluorogen activating peptides again, so I went back and looked at this data again, and it really isn’t bad.
![](https://i0.wp.com/www.matreyeklab.com/wp-content/uploads/F107.png?resize=451%2C353&ssl=1)
![](https://i0.wp.com/www.matreyeklab.com/wp-content/uploads/F109.png?resize=768%2C267&ssl=1)
![](https://i0.wp.com/www.matreyeklab.com/wp-content/uploads/F111.png?resize=768%2C431&ssl=1)
In summary. 1) Works well, giving ~ 100-fold increase to fluorescence from background when highly expressed. 2) Probably want to do at least 500 ng/mL or perhaps even 1ug/mL to improve signal over background. 3) Still get specific signal after washout, but you may as well leave it on and get max signal.