Some Expected Yields

Posted on by kmatreyek

Here is some real-world data describing expected yields we may expect from some of these routine lab procedures or services.

Obviously the above plot is about how much total plasmid DNA we get from the miniprep kit we use in the lab.
The plot above show the expected total yields of DNA based on the extraction type / method
And this is the pretty wide range of reads we’ve gotten from submitting plasmids to plasmidsaurus
The above graph shows how many (raw) reads we’ve gotten from Azenta / Genewiz Amplicon-EZ.

Oh, and this is a good one:

How well my determination of flask “confluency” actually correlated with cell counts. I mean, sure, there must be some error being imparted by the actual measurement of the cells when counting, but I think we all know it’s mostly that my estimate really isn’t precisely informative.